[Sunflower]

Zu den "viable but non culturable bacteria" , also "lebenden, aber nicht kultivierbaren Bakterien" ist mir Folgendes aufgefallen.

In Tierstudien wurden nicht kultivierbare Borrelien nach Antibiosen aus dem Gewebe der behandelten Tiere gefunden, z.B. in dieser Studie:

PLoS One. 2014 Jan 23;9(1):e86907. doi: 10.1371/journal.pone.0086907. eCollection 2014.

Resurgence of persisting non-cultivable Borrelia burgdorferi following antibiotic treatment in mice.

Hodzic E, Imai D, Feng S, Barthold SW.

http://www.ncbi.nlm.nih.gov/pubmed/24466286

Experimental studies in dogs, mice, and non-human primates have found persistence of B. burgdorferi DNA following treatment with a variety of antibiotics, but persisting spirochetes are non-cultivable(...)Results confirmed previous studies, in which B. burgdorferi could not be cultured from tissues, but low copy numbers of B. burgdorferi flaB DNA were detectable in tissues at 2, 4 and 8 months after completion of Treatment.(...)Despite the continued non-cultivable state, RNA transcription of multiple B. burgdorferi genes was detected in host tissues, flaB DNA was acquired by xenodiagnostic ticks, and spirochetal forms could be visualized within ticks and mouse tissues by immunofluorescence and immunohistochemistry, respectively.

Nach den Antibiosen haben die Forscher DNA von Bb in Gewebe von den infizierten und behandelten Tiere und sie konnten sogar spirochetale Formen im Gewebe der Tiere entdecken.Wie in anderen Studien waren die Bakterien aus dem Gewebe der Tiere nicht kultivierbar.

In der Studie von Dr.Oliver steht:

The Viable but Nonculturable State in Bacteria

James D. Oliver

Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223-0001, USA
(Accepted September 13, 2004)

http://www.msk.or.kr/jsp/view_old_journa...erSeq=2134

It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow.
This mini-review defines what the “viable but nonculturable” (VBNC) state is, and illustrates the methods that can be used to show that a bacterial cell is in this physiological state. The diverse environmental factors which induce this state, and the variety of bacteria which have been shown to enter into the VBNC state, are
listed. In recent years, a great amount of research has revealed what occurs in cells as they enter and exist in this state, and these studies are also detailed. The ability of cells to resuscitate from the VBNC
state and return to an actively metabolizing and culturable form is described, as well as the ability of these cells to retain virulence. Finally, the question of why cells become nonculturable is addressed. It
is hoped that this mini-review will encourage researchers to consider this survival state in their studies as an alternative to the conclusion that a lack of culturability indicates the cells they are examining are
dead.

Nicht kultivierbare Bakterien sind also nicht tot, sondern können in einen metabolisch aktiven Zustand zurückkehren, sich also wieder teilen.

Sind also Borrelien, die man in Tieren nach Antibiosen entdeckt hat, die aber nicht kultivierbar waren, "VBCN" Bakterien? Lebten diese Bakterien in Biofilmen in diesem Ruhezustand?

Wenn ja, dann würde es bedeuten, dass diese nicht kultivierbaren Bakterien lebend waren und somit pathologisch und dass Biofilme für die Antibiotikaresistenz bei Tieren mit Borreliose verantwortlich wären.